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Thermo Fisher
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Bio-Techne corporation
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Proteintech
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Cell Signaling Technology Inc
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Recombinant protein of human MID1 interacting protein 1 gastrulation specific G12 homolog zebrafish MID1IP1 transcript variant 1
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MID1IP1 MS Standard C13 and N15 labeled recombinant protein NP 067065
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Human MID1IP1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. AcceGen offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: The Antitumor Effect of Timosaponin A3 through c-Myc Inhibition in Colorectal Cancer Cells and Combined Treatment Effect with 5-FU or Doxorubicin
doi: 10.3390/ijms231911900
Figure Lengend Snippet: TA3 inhibits c-Myc and induces apoptosis via CNOT2 or MID1IP1. ( A ) HCT116 p53+/+ cells were transfected with CNOT2 siRNA or control siRNA for 48 h and then cells were treated with TA3 (12.5 μM) for 24 h (three independent experiments in HCT116 p53+/+ cells). ( B ) HCT116 p53+/+ cells were transfected with MID1IP1 siRNA or control siRNA for 48 h and then cells were treated with TA3 for 24 h (three independent experiments in HCT116 p53+/+ cells). Statistical significance was analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s test and the unpaired t-test. p -value indicates a significant difference between the groups. Quantification of protein expression is shown in the lower panel. * p < 0.01, ** p < 0.005, *** p < 0.001 vs. control; # p < 0.01, ## p < 0.005, ### p < 0.001 vs. only TA3-treated group. ( C ) HCT116 p53+/+ cells were treated with TA3 for 24 h. Then, cells were harvested for co-immunoprecipitation assay.
Article Snippet: Primary antibodies for CNOT2 (Cat No. 34214) and PARP (Cat No. 9542) were purchased from Cell Signaling Technology (Beverly, MA, USA),
Techniques: Transfection, Control, Expressing, Co-Immunoprecipitation Assay
Journal: International Journal of Molecular Sciences
Article Title: The Antitumor Effect of Timosaponin A3 through c-Myc Inhibition in Colorectal Cancer Cells and Combined Treatment Effect with 5-FU or Doxorubicin
doi: 10.3390/ijms231911900
Figure Lengend Snippet: Mechanism of TA3 to induce apoptosis in colorectal cancer by downregulating c-Myc via CNOT2 or MID1IP1.
Article Snippet: Primary antibodies for CNOT2 (Cat No. 34214) and PARP (Cat No. 9542) were purchased from Cell Signaling Technology (Beverly, MA, USA),
Techniques: